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This research examines how 20-hydroxyecdysone influences molting regulation in crayfish.

March 8, 2026BiologyOpen Access

Transcriptomic and Microbiome Analyses of Procambarus clarkii Exposed to Different Doses of 20E

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Authors

YZYan ZouMarine Biology Institute of Shandong Province CZChen-Yang ZhangShandong Agricultural University XCXiao-Tong CaoShandong Agricultural University

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Implication

Transcriptomic analyses reveal that 20-hydroxyecdysone affects molting and microbiome in Procambarus clarkii, indicating dysbiosis and enhanced proteolysis.

Key Points

This research examines how 20-hydroxyecdysone influences molting regulation in crayfish.
Injected Procambarus clarkii with different doses of 20-hydroxyecdysone (20E)
Conducted transcriptomic analyses to identify differentially expressed genes (DEGs)
Analyzed microbiome changes in response to 20E treatment
20E induced 13,253 unique DEGs in the epidermis, enriching pathways related to proteolysis
250 ng/g 20E resulted in 2395 DEGs in epidermis and 99 in hemocytes, focusing on metabolic and mitochondrial pathways
20E stimulation led to dysbiosis, notably affecting Escherichia-Shigella and Vibrio species

Cite This Study

Zou et al. (2026) studied this question.

synapsesocial.com/papers/69acc5b032b0ef16a4050434 https://doi.org/10.3390/biology15050434

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Implication

Transcriptomic analyses reveal that 20-hydroxyecdysone affects molting and microbiome in Procambarus clarkii, indicating dysbiosis and enhanced proteolysis.

Transcriptomic and Microbiome Analyses of Procambarus clarkii Exposed to Different Doses of 20E

Discussion

Member takes

Implication

Key Points

Cite This Study

Transcriptomic and Microbiome Analyses of Procambarus clarkii Exposed to Different Doses of 20E

Discussion

Member takes

Implication

Key Points

Cite This Study

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