Chromosome-level genome assembly of Neoseiulus longispinosus (Phytoseiidae)

Neoseiulus longispinosus is a predatory mite (Phytoseiidae) widely used in the biological control of agricultural pests, such as Tetranychus mites, Eriophyes mites and Oligonychus mites, in tropical and subtropical regions in China. It shows strong adaptability to tropical climates and high temperatures. To explore the molecular basis of its excellent properties, such as high-temperature resistance, and to promote its practical application, we conducted a genome sequencing study on N. longispinosus. Here, we report a chromosome-level genome assembly and annotation of the N. longispinosus genome using multiplatform sequencing. The final assembly spanned 199.21 Mb, close to the 210.09 Mb estimated by 21-mer analysis. The chromosome-level assembly achieved a scaffold N50 of 54.56 Mb and a contig N50 of 2.35 Mb. Hi-C scaffolding anchored 98.59% of the assembled sequences to four pseudochromosomes. Genome annotation identified 22.38% repetitive elements and 12,890 predicted genes in the genome. This high-quality genome provides a robust reference for future studies on biological control employing predatory mites.