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March 12, 2026Human Reproduction OpenOpen Access

Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue in vitro maturation enhances maturation rates and modulates oocyte proteome and ultrastructure

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Authors

SMSofia MakievaUniversity Hospital of ZurichMDMara D Saenz- de-JuanoETH ZurichCACarmen AlmiñanaUniversity Hospital of Zurich

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Overview

Demonstrates the benefits of using extracellular vesicles to enhance oocyte maturation, indicating potential for improved fertility treatments.

Key Points

  • The research aims to investigate the impact of follicular fluid-derived extracellular vesicles on human oocyte in vitro maturation.
  • Isolated extracellular vesicles from human follicular fluid through ultracentrifugation.
  • Analyzed protein content of vesicles using mass spectrometry.
  • Assessed uptake of fluorescently-labelled vesicles by immature oocytes via confocal microscopy.
  • Cultured immature oocytes with or without vesicles for maturation and assessed maturation rates through polar body extrusion.
  • Conducted proteomic analysis of matured oocytes and observed organelle structure via transmission electron microscopy.
  • Supplementation of maturation culture with extracellular vesicles improved oocyte maturation rates by 22.8%.
  • Identified 1340 unique proteins in the extracellular vesicles, with significant enrichment in MII-derived vesicles.
  • Differential protein analysis revealed 56 proteins with varying abundance between supplemented and non-supplemented oocytes, including key maturation-related proteins.
  • Electron microscopy showed structural differences in organelles, particularly endoplasmic reticulum.

Cite This Study

Makieva et al. (2026) studied this question.

synapsesocial.com/papers/69b25b2b96eeacc4fcec9a00https://doi.org/10.1093/hropen/hoag021
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Treatment of human oocytes with extracellular vesicles from follicular fluid during rescue <i>in vitro</i> maturation enhances maturation rates and modulates oocyte proteome and ultrastructure2026
  2. 2O-005 The in vitro maturation rate of human oocytes is enhanced following uptake of extracellular vesicles derived from mature follicles2024 · 1 citations
  3. 3Extracellular vesicles from the follicular fluid of competent oocytes improve blastocyst yields when supplemented during simulated physiological oocyte maturation in cattle2025 · 5 citations
  4. 4Follicular fluid-derived small extracellular vesicles during individual in vitro maturation improve blastocyst development2025
  5. 5P-795 Robust and simple in vitro maturation of ovarian tissue oocytes2024