What question did this study set out to answer?

The aim is to develop a reliable RT-qPCR assay for the detection and typing of the newly emerging Bluetongue virus serotype 5 from field samples.

March 13, 2026

Development and validation of a real time RT-qPCR assay for detection of the emerging Bluetongue virus serotype 5 from field samples.

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Development and validation of a real-time RT-qPCR assay detects Bluetongue Virus serotype 5, showing its effectiveness in managing outbreaks.

The aim is to develop a reliable RT-qPCR assay for the detection and typing of the newly emerging Bluetongue virus serotype 5 from field samples.
Developed and validated a real-time RT-qPCR assay
Optimised for high sensitivity and specificity
Conducted testing on field samples for BTV-5 ITA 2025 detection and typing
Used Whole Genome Sequencing for initial strain identification
The assay demonstrated high sensitivity and specificity
Validated for effective detection of BTV-5 in field samples
Supported improvement of surveillance and control strategies for Bluetongue outbreaks

Palombieri et al. (2026) studied this question.

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Development and validation of a real-time RT-qPCR assay detects Bluetongue Virus serotype 5, showing its effectiveness in managing outbreaks.