Identification and functional characterization of key ecdysteroid biosynthesis and signalling genes regulating moulting and metamorphosis in Dastarcus helophoroides
Comparative analysis identifies gene roles in moulting and metamorphosis in Dastarcus helophoroides, suggesting growth regulation strategies.
Key Points
The aim is to identify and characterize genes involved in ecdysteroid biosynthesis and signalling related to moulting and metamorphosis in Dastarcus helophoroides.
Conducted comparative transcriptome analysis during larval development.
Validated gene expression patterns using RT-qPCR.
Applied RNA interference (RNAi) to silence key genes affecting development.
Identified eight key genes linked to ecdysteroid biosynthesis and signalling.
RNAi treatment caused a 47.83-100% reduction in adult eclosion.
Observed morphological abnormalities, including pigmentation and wing deformities.