This research uncovers a simplified method to assess photolytic efficiency in phosphoramidite compounds, highlighting implications for chemical reactions.
Light-sensitive protecting chemical groups play an important role in the control of chemical reactions with high spatial and temporal resolution. Various forms of o-nitrobenzyl as a protecting compound are used for light-directed DNA synthesis. The suitability of a particular derivative for the application is defined by its photolytic efficiency, a characteristic, that is commonly extracted from a repetitive HPLC procedure. Here, using an example of a phosphoramidite compound with improved properties of deprotection, we delineate a simplified and economic approach based on a measurement of absorbance spectra to evaluate the photolytic efficiency. The obtained values are in close agreement with those determined previously.
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Karpicz et al. (2026) studied this question.
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