Objectives: We investigated the concentration-dependent cytotoxic effects of Marrubium alysson L. extract on HNO210 human laryngeal carcinoma cells. Methods: The HNO210 human laryngeal carcinoma cell line (BHC11100312, BioHippo) was cultured in Dulbecco’s Modified Eagle Medium (DMEM) (ATCC, 30-2002). Seven working concentrations (10–500 μL/mL) of Marrubium alysson L. were prepared. The antiproliferative effect was assessed using the colorimetric MTT assay. Results: Marrubium alysson L. inhibits the viability of HNO210 cells. The corresponding logIC₅₀ (2.681 μL/mL), along with a high coefficient of determination (R²=0.9960), confirms the reliability of the dose-response relationship. While lower concentrations largely preserve cell viability, higher doses significantly suppress metabolic activity. Marrubium alysson L. extract demonstrates a concentration-dependent cytotoxic effect on HNO210 cells. After 24 hours of exposure, treated cells showed notable morphologic changes, including cell shrinkage, rounding, and detachment from the culture surface, indicating loss of membrane integrity and decreased adherence. Conclusion: Marrubium alysson L. inhibits the viability of HNO210 laryngeal carcinoma cells. It exerts a concentration-dependent cytotoxic effect and anticancer potential against these cells. It affects cell morphology and reduces adherence. Marrubium alysson L. has potential as a source of bioactive compounds with antiproliferative activity for designing highly effective anticancer drugs.
Coskun et al. (2026) studied this question.