A Customizable, Low-cost 3D-Printed Device for Live Cell Confinement Imaging

Key Points

• This research aims to develop a low-cost, customizable device for live cell confinement imaging to examine how physical constraints influence cell behavior.
• Designed a 3D-printed device for live cell confinement.
• Enabled simultaneous imaging of cells under confinement conditions.
• Evaluated the impact of confinement on cell migration and cancer invasiveness.
• The device effectively replicated physical constraints on cell behavior.
• Cells showed altered migration patterns when confined.
• Cancer cell invasiveness was significantly affected by confinement conditions.