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This protocol aims to enhance the detection and isolation of Aspergillus terreus from soil samples.

March 21, 2026BIO-PROTOCOLOpen Access

Soil Extraction Plating for the Detection of Aspergillus terreus from Environmental Samples

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This protocol aims to enhance the detection and isolation of Aspergillus terreus from soil samples.
Sieved soil is suspended in saline-surfactant buffer to release fungal propagules.
Supernatant is plated onto selective media with chloramphenicol and amphotericin B.
Incubation is performed at varying temperatures to favor thermotolerant strains.
Colony identification is conducted using microscopy and sequencing.
Enhanced recovery of A. terreus from soil samples was achieved through selective plating.
Differential incubation temperature effectively supported thermotolerant A. terreus growth.
Use of chloramphenicol effectively suppressed bacterial contaminants.

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Protocol demonstrates selective isolation of Aspergillus terreus from environmental samples, implying improved detection methods.

Schobert et al. (2026) studied this question.

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