Study of Biological Activities of Microporus xanthopus and Phylloporia pectinata From Gulmi, Nepal

Mushrooms are recognized for their therapeutic qualities in addition to their nutritional value. Numerous substances with therapeutic properties are present even in inedible mushrooms. These substances can be used to make potent medications once they have been refined and examined. The present study aims to explore the biological activities of the extracts of Microporous xanthopus and Phylloporia pectinata , collected from Gulmi District, Nepal. The methanolic and ethyl acetate extracts of both species were subjected to phytochemical screening, total phenolic content (TPC), total flavonoid content (TFC), and antioxidant and antibacterial activity. The LC–MS analysis was performed to identify the compounds present in the extract by detecting and analyzing their molecular ion peaks. The TPC (611.89 and 319.16 mg GAE/g) and TFC (57.18 and 142.37 mg QE/g) were found highest in the ethyl acetate extract of P. pectinata and M. xanthopus, respectively. The antioxidant activity (DPPH radical scavenging %) of the methanolic extract and the ethyl acetate extract of M. xanthopus was found to be 41.7% and 21.6%, whereas that for P. pectinata was found to be 78.6% and 55.4%, respectively. Those extracts were found to inhibit the growth of S. aureus, E. coli , and S. typhi . The LC–MS analysis of the extract of P. pectinata showed eight important secondary metabolites present in the extract. These mushrooms, with their rich content of bioactive compounds, demonstrate significant antioxidant and antibacterial properties, indicating their potential for medicinal applications.