What is the Cellular Function under High Pressure Conditions?

Homeostasis in living organisms is regulated by mechanical factors, including pressure. However, the mechanisms by which pressure influences cellular functions remain unclear, partly due to methodological limitations. Here, we describe a system capable of applying controlled hydrostatic pressure to cultured cells and of evaluating their responses. Under high-pressure conditions, we observed alterations in cell morphology, subcellular protein localization, and gene expression profiles. These findings provide new insights into pressure-induced cellular regulation and demonstrate the utility of our system as a platform for elucidating mechanobiological processes mediated by pressure stimuli.