Laboratory experiment teaches students to analyze DNA sequences using software and online resources, indicating hands-on learning in biochemistry.
The purpose of this laboratory experiment is to teach undergraduate students how to use a commercial software program and Internet resources to analyze DNA sequences. Several primers were designed for DNA sequencing of the xylose reductase gene so that students can learn to reconstruct the gene sequence by linking and aligning fragmented sequences with the help of DNASTAR’s Lasergene software. This program will also allow students to determine the start and termination of the gene sequence, edit and annotate the sequence, and present the DNA sequence in the widely used GenBank format. Students will learn the procedures for comparing their sequences with published DNA data available via the Internet and determining the exact sizes of the amplified fragments by mapping the locations of the primers that are used for making the PCR fragments.
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Beng Guat Ooi (2005) studied this question.
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