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March 28, 2026The Journal of Physical Chemistry B

Transformation Process Analysis of Amorphous Calcium Phosphate to Hydroxyapatite by In Situ Fluorescence Dual Probe

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Authors

XTXi TanCommunication University of ZhejiangJCJianzhong ChenJiangnan UniversityYGYating GaoWuhan University of Technology

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Implication

In situ fluorescence analysis shows the ACP-HAP transformation in a cycling system, suggesting insights into biomineralization.

Key Points

  • This research aims to characterize how amorphous calcium phosphate transforms to hydroxyapatite with the help of polyacrylic acid.
  • Developed an in situ fluorescence dual probe method using Eu<sup>3+</sup> and TCPE.
  • Analyzed the fluorescence ratio of Eu<sup>3+</sup> to visualize crystallization features.
  • Measured TCPE fluorescence intensity to monitor polyacrylic acid's state during transformation.
  • The Eu<sup>3+</sup> fluorescence ratio first decreased then increased during crystallization.
  • TCPE fluorescence intensity consistently decreased, indicating the transfer of Ca<sup>2+</sup> ions.
  • Quantitative analysis revealed HAP content rising to approximately 3.288% by day four in a cycling system.

Cite This Study

Tan et al. (2026) studied this question.

synapsesocial.com/papers/69c7722a8bbfbc51511e2637https://doi.org/10.1021/acs.jpcb.5c08376
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