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March 29, 2026Journal of Nuclear Medicine0 citationsOpen Access

Molecular Imaging Techniques for Collagen Turnover in Myocardial Infarction

Molecular Imaging of Collagen Turnover in Myocardial Infarction

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Population

10- to 16-week-old C57BL/6J mice with induced myocardial infarction, and ex vivo human cardiac tissue

Comparison

99mTc-3-9 radiotracer for SPECT/CT imaging vs Sham-operated mice, remote non-infarcted…

Design

Preclinical

Follow-up

Up to 2 weeks

Key result

99mTc-(HE)3-(GPO)9 SPECT/CT imaging revealed significantly higher tracer uptake (0.65 %ID/mL) in the infarct zone of mice 2 weeks post-myocardial infarction compared to sham controls (0.34 %ID/mL).

Authors

ANAfarin NeishabouriMGMean GhimOVOnur Varli

Discussion

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Overview

May serve as noninvasive biomarker for post-MI remodeling; hypothesis-generating for clinical translation of collagen-targeted SPECT.

Key Points

  • The aim is to assess the effectiveness of a novel radiotracer in detecting collagen turnover after myocardial infarction.
  • Induction of myocardial infarction in mice via artery ligation
  • Use of SPECT/CT imaging for tracer evaluation in infarcted areas
  • Comparison of tracer uptake with a control tracer
  • Ex vivo analysis of tissue using autoradiography and specific imaging techniques
  • Significantly higher tracer uptake in the infarct zone compared to remote zones and controls (P < 0.0001)
  • Strong correlation between SPECT and autoradiography findings (ρ = 0.81, P < 0.05)
  • Recognition of denatured collagen in human fibrotic cardiac tissue
  • Increased ratio of denatured collagen to procollagen from day 3 to week 2 after MI

Structured PICO

P
Population
10- to 16-week-old C57BL/6J mice with induced myocardial infarction, and ex vivo human cardiac tissue
I
Intervention
99mTc-(HE)3-(GPO)9 radiotracer for SPECT/CT imaging
C
Comparator
Sham-operated mice, remote non-infarcted myocardium, and a scrambled control tracer (99mTc-(HE)3-(GPO)SCR)
O
Outcome
Tracer uptake in the infarct zone compared to remote zone and sham controlssurrogate

99mTc-(HE)3-(GPO)9 enables noninvasive molecular imaging of denatured collagen, providing a potential biomarker for active collagen turnover and cardiac remodeling after myocardial infarction.

Main Result

Absolute Event Rate: 0.65% vs 0.34%

p-value: p=<0.0001

Limitations

  • Long-term changes in collagen denaturation and functional significance of the signal remain to be addressed
  • Effect of antifibrotic therapies on the CHP signal is unknown
  • Small size of the murine left ventricle limited evaluation of regional changes
  • Nonspecific liver uptake of the tracer

Cite This Study

Neishabouri et al. (2026) studied Myocardial Infarction (n=47). 99mTc-(HE)3-(GPO)9 vs. Sham surgery was evaluated on Tracer uptake in the left ventricular wall by SPECT/CT (%ID/mL) (p=<0.0001). 99mTc-(HE)3-(GPO)9 SPECT/CT imaging revealed significantly higher tracer uptake (0.65 %ID/mL) in the infarct zone of mice 2 weeks post-myocardial infarction compared to sham controls (0.34 %ID/mL).

synapsesocial.com/papers/69c8c15ade0f0f753b39bda9https://doi.org/10.2967/jnumed.125.271721
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