What question did this study set out to answer?

The aim is to determine whether lipidation of the PKKKRKV peptide can improve its antimicrobial properties and explore the resulting structure-activity relationships.

April 3, 2026Artificial Cells Nanomedicine and BiotechnologyOpen Access

Lipidation of a nuclear localization sequence enhances broad-spectrum antimicrobial activity: synthesis, characterization, and in silico analysis

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Authors

RMRani MaharaniPadjadjaran University TFTaufik Muhammad FakihBandung Islamic University MMMuhamad Imam MuhajirPadjadjaran University

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Implication

Evaluates lipidation effects on antimicrobial activity in a cationic peptide, suggesting a potential strategy for broad-spectrum agents.

Key Points

The aim is to determine whether lipidation of the PKKKRKV peptide can improve its antimicrobial properties and explore the resulting structure-activity relationships.
Synthesis of five PKKKRKV-based lipopeptides using Fmoc solid-phase peptide synthesis.
Conjugation of lipopeptides with various fatty acids (C16-C18).
Purification of compounds by RP-HPLC and characterization via mass spectrometry and NMR.
Assessment of antimicrobial activity against various bacteria and fungi using broth microdilution assays.
Conduct of in silico studies including molecular docking and dynamics simulations.
Lipidation significantly enhanced antimicrobial activity of the peptides.
LP-5 (C18:1, unsaturated) showed the broadest spectrum with MIC values of 11.59-23.19 µM against Gram-positive and Gram-negative bacteria.
LP-2 (C16:0, saturated) was selectively potent against E. coli with an MIC of 6.96 µM.
LP-4 and LP-5 exhibited small HOMO-LUMO gaps (∼0.111-0.112 eV), indicating higher electronic reactivity.

Cite This Study

Maharani et al. (2026) studied this question.

synapsesocial.com/papers/69cf5dd55a333a821460bd10 https://doi.org/10.1080/21691401.2026.2649071

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April 3, 2026Artificial Cells Nanomedicine and BiotechnologyOpen Access

Lipidation of a nuclear localization sequence enhances broad-spectrum antimicrobial activity: synthesis, characterization, and in silico analysis

View Full Paper

Ask AI

Bookmark

Authors

RMRani MaharaniPadjadjaran University TFTaufik Muhammad FakihBandung Islamic University MMMuhamad Imam MuhajirPadjadjaran University

Discussion

Member takes

Implication

Evaluates lipidation effects on antimicrobial activity in a cationic peptide, suggesting a potential strategy for broad-spectrum agents.

Key Points

The aim is to determine whether lipidation of the PKKKRKV peptide can improve its antimicrobial properties and explore the resulting structure-activity relationships.
Synthesis of five PKKKRKV-based lipopeptides using Fmoc solid-phase peptide synthesis.
Conjugation of lipopeptides with various fatty acids (C16-C18).
Purification of compounds by RP-HPLC and characterization via mass spectrometry and NMR.
Assessment of antimicrobial activity against various bacteria and fungi using broth microdilution assays.
Conduct of in silico studies including molecular docking and dynamics simulations.
Lipidation significantly enhanced antimicrobial activity of the peptides.
LP-5 (C18:1, unsaturated) showed the broadest spectrum with MIC values of 11.59-23.19 µM against Gram-positive and Gram-negative bacteria.
LP-2 (C16:0, saturated) was selectively potent against E. coli with an MIC of 6.96 µM.
LP-4 and LP-5 exhibited small HOMO-LUMO gaps (∼0.111-0.112 eV), indicating higher electronic reactivity.

Cite This Study

Maharani et al. (2026) studied this question.

synapsesocial.com/papers/69cf5dd55a333a821460bd10 https://doi.org/10.1080/21691401.2026.2649071

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