The Effect of Inflammatory Activation on Mitochondrial Function and Expression of Apoptosis Inhibitor Genes in THP-1 Acute Myeloid Leukemia Cells

A study was conducted on the key functions of mitochondrial respiration and the expression of genes of the BCL2 and IAP families in THP-1 cells of acute myeloid leukemia upon lipopolysaccharide and zymosan A-induced inflammatory activation, as well as under conditions of aseptic inflammatory activation simulating the inflammatory microenvironment using three-dimensional cell cultures. It was found that under conditions of both lipopolysaccharide and zymosan A-induced as well as aseptic inflammatory activation, a decrease in mitochondrial membrane potential and the rate of oxygen consumption in mitochondria occurred along with increased secretion of proinflammatory mediators IL-1β, TNF-α and IL-6. In addition, an increase in the production of reactive oxygen species and lactate was shown. It was also shown that in THP-1 cells, under conditions of inflammatory activation, there was an increase in the expression of the BIRC3, BIRC7, BCL2, BCL2L1, BCL2L2, and BCL2A1 genes encoding the corresponding antiapoptotic proteins.