Abstract 741: Illuminate your targets: Endogenous HiBiT knock-in monoclones—inventory + rapid custom.

Key Points

• The research aims to streamline protein quantification through the development of HiBiT knock-in monoclones for various applications.
• Developed a platform with genome-integrated LgBiT and CRISPR-Cas9 HDR for precise HiBiT insertions.
• Established monoclonal cell lines with over 30 tagged genes related to oncogenic signaling and transcriptional regulation.
• Utilized standardized NanoLuc assays for rapid quantitative analysis of protein degradation kinetics.
• Successfully created a diverse portfolio of HiBiT-KI monoclonal cell lines for major target classes.
• Reduced assay setup time from weeks to days, improving efficiency.
• Demonstrated consistent quantitative output across different targets and experimental conditions.