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May 23, 2026PlasmidOpen Access

Cell lines stably expressing iBiT constructs enabled highly reproducible, longitudinal, and time-resolved analyses of protein-protein interactions.

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Why the study?

NanoLuc Binary Technology reliability depends critically on balanced expression of interacting proteins, requiring a system to ensure reproducible and finely controlled expression without overexpression artifacts.

Population

Human cell lines

Comparison

Full-length isoform 1 vs N-terminally truncated isoform 2 of p62/Sequestosome 1 interaction

Design

Preclinical laboratory method development study

Authors

JDJan DreuteMSM. Lienhard Schmitz

Discussion

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Overview

May aid live-cell PPI detection; leaves open broader validation before wider adoption.

Structured PICO

P
Population
Intact human cell lines (targeting safe harbor locus on human chromosome 19)
I
Intervention
Inducible binary technology (iBiT) system (plasmid system for stable and biallelic integration of NanoLuc plasmids regulated by Tet-On system)
O
Outcome
Measurement of protein-protein interaction dynamics (specifically between p62/Sequestosome 1 isoforms 1 and 2)

The iBiT system provides a robust platform for assessing protein-protein interaction dynamics and their pharmacological modulation in intact cells without overexpression artifacts.

Cite This Study

Dreute et al. (2026) studied this question.

synapsesocial.com/papers/6a6f8e4af44fa9f079dd01b4https://doi.org/10.1016/j.plasmid.2026.102772
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1In Vivo Detection of Metabolic Fluctuations in Real Time Using the NanoBiT Technology Based on PII Signalling Protein Interactions2024 · 4 citations
  2. 2Development of a global screening system for detecting protein–protein interactions by luminescence complementation in fission yeast2026
  3. 3<scp>MemPPI</scp> platform for measuring and engineering membrane protein–protein interactions in mammalian cells via split nanoluciferase2026 · 1 citations
  4. 4A Modular Platform for Quantitative Two-Color Bioluminescence Combining NanoLuc and Red-Shifted NanoPrism Luciferases2026 · 1 citations
  5. 5Abstract 741: Illuminate your targets: Endogenous HiBiT knock-in monoclones—inventory + rapid custom.2026