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February 14, 2026Protein ScienceOpen Access

MemPPI platform for measuring and engineering membrane protein–protein interactions in mammalian cells via split nanoluciferase

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Authors

WTWeiyi TangScripps Research InstituteSJSebastian Jojoa‐CruzScripps Research InstituteJLJiayi LiScripps Research Institute

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Overview

This platform quantifies membrane protein interactions in human cells, suggesting improvements for protein engineering and drug discovery.

Key Points

  • The study aims to develop a reliable platform for measuring membrane protein-protein interactions in mammalian cells.
  • Developed split luciferase complementation NanoBit system for quantification.
  • Created expression constructs for diverse membrane protein interactions.
  • Measured and normalized protein expression levels in cells.
  • Tested assay robustness with various memPPI pairs and topologies.
  • Established a high dynamic range for detecting true interactions.
  • Identified suitable assay conditions that distinguish positive interactions from noise.
  • Enhanced tracking and expression control for membrane proteins.

Cite This Study

Tang et al. (2026) studied this question.

synapsesocial.com/papers/6990113f2ccff479cfe57c5ehttps://doi.org/10.1002/pro.70496
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