Distinct dendritic cell cytoskeletal programs dictate synapse architecture and CD8+ T cell fate

Dendritic cell activation of CD8 + T cells at the immunological synapse is critical for immunity, but the structural organization of the dendritic cell side and its impact on T cell fate remain poorly defined. Using bone marrow-derived dendritic cells (BMDCs) as a model, we describe two stable subpopulations distinguished by their capacity to form morphologically distinct synapses. We demonstrate that this architectural divergence is governed by the differential expression of the co-stimulatory molecule CD70: CD70 high BMDCs form spiky “firework” synapses driven by a filopodia-based cytoskeletal program, while CD70 low BMDCs form smooth “pancake” synapses. This structural dichotomy functionally dictates T cell programming. CD70 high dendritic cells prime potent, terminally differentiated Tc1 effector cells. In contrast, IL-6-secreting CD70 low dendritic cells generate memory T cells with a Tc17-like functional profile and robust recall capacity. Our work reveals that DC synapse architecture is a key determinant of T cell fate, linking the physical organization of the cell to distinct immunological outcomes.