Abstract 4830: Mic60-low breast cancer cells activate epithelial-mesenchymal transition and support aggressive tumor growth

Key Points

• The aim is to investigate the role of Mic60 in modulating epithelial-mesenchymal transition and enhancing tumor aggressiveness in breast cancer.
• Silenced Mic60 gene using siRNA and shRNA in breast cancer cell lines.
• Analyzed gene expression via RT-qPCR and protein levels using Western blotting.
• Assessed cell migration and invasion through transwell assays.
• Evaluated in vivo tumorigenic potential in immunocompetent mice.
• Mic60 silencing increased expression of EMT master regulators and altered adhesion molecule profiles.
• Enhanced migration and invasion capabilities were observed in vitro.
• Silenced cell lines exhibited accelerated tumor growth when injected in vivo.