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This study aims to create and validate a CyTOF panel to examine macrophage polarization and the effects of treatments on these immune cells.

Development of a CyTOF Panel for Macrophage Polarization and Functional Assessment

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This study aims to create and validate a CyTOF panel to examine macrophage polarization and the effects of treatments on these immune cells.
Developed a 40-marker CyTOF panel focused on macrophage and immune markers.
Induced macrophage polarization in vitro using RAW 264.7 cell line with various stimuli.
Utilized a mouse model of osteosarcoma to evaluate treatment effects on TAMs.
Analyzed tumor samples using dimensionality reduction and clustering algorithms.
The CyTOF panel effectively identified macrophage subtypes in both in vitro and in vivo settings.
In vitro results showed distinct phenotypic clusters for M1 and M2 subtypes.
Treatment led to increased M1 marker expression and reduced M2 markers, indicating a shift in polarization.

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This research develops a CyTOF panel to assess macrophage polarization and immune markers in tumor environments, suggesting new treatment strategies.

Gourabathini et al. (2026) studied this question.

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This research develops a CyTOF panel to assess macrophage polarization and immune markers in tumor environments, suggesting new treatment strategies.