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This research investigates how cancer cell-derived factors influence the behavior of monocyte-derived macrophages in co-culture settings.

Decoding Interactions Between Cancer Cells and Macrophages Using Flow Cytometry and Immunoassays

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This research investigates how cancer cell-derived factors influence the behavior of monocyte-derived macrophages in co-culture settings.
Cultured THP1-derived macrophages with conditioned medium from colon, lung, and breast cancer cell lines.
Characterized macrophages using flow cytometry for surface markers and soluble factors.
Activated and polarized macrophages into M1 and M2 phenotypes before co-culturing with cancer cells.
Measured cancer cell proliferation and viability using flow cytometry.
Cancer cells release soluble factors that impact macrophage phenotypes.
M1 macrophages promote anti-tumor activity, while M2 macrophages support cancer cell proliferation.
Differences in viability and proliferation of cancer cells were observed depending on the macrophage subtype.

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In vitro study reveals how cancer cells influence macrophage phenotypes and their effects on cancer cell viability.

Zhao et al. (2026) studied this question.

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