Structural and functional characterization of the coxsackievirus B3 CRE(2C): role of CRE(2C) in negative- and positive-strand RNA synthesis

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Population

Coxsackievirus B3 (CVB3) genome and HeLa S10 cell extracts

Comparison

Site-directed mutagenesis and disruption of the… vs Wild-type CVB3 CRE(2C)

Design

Preclinical

Authors

Discussion

CVB3 CRE(2C) is required for efficient RNA synthesis; hypothesis-generating for antiviral targets in myocarditis models.

Population

Coxsackievirus B3 (CVB3) genome and HeLa S10 cell extracts

Intervention

Site-directed mutagenesis and disruption of the CRE(2C) stem-loop element

Comparator

Wild-type CVB3 CRE(2C)

Outcome

Viral RNA replication and positive/negative-strand RNA synthesissurrogate

The CVB3 CRE(2C) element is essential for the initiation of both positive- and negative-strand viral RNA synthesis.

Ooij et al. (2005) studied this question.

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