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November 15, 2000Journal of VirologyOpen Access

Genetic and Biochemical Studies of Poliovirus cis -Acting Replication Element cre in Relation to VPg Uridylylation

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Population

Poliovirus type 1 and type 3 cre elements (in vitro and in vivo models)

Comparison

Mutations in the cre element vs Wild-type sequence

Design

Preclinical

Authors

ERElizabeth RiederNational Agricultural Statistics ServiceAPAniko V. PaulState University of New YorkDKDong Wook KimHanyang University

Discussion

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Implication

Disrupts PV-cre function and abolishes infectivity; leaves open whether analogous elements are essential across human enteroviruses.

Structured PICO

P
Population
Poliovirus type 1 and type 3 cre elements (in vitro and in vivo models)
I
Intervention
Mutations in the cre element (e.g., A4472C, A4473C, A4474G)
C
Comparator
Wild-type sequence
O
Outcome
VPg uridylylation reaction and replication phenotypes in vivo (RNA infectivity, virus yield)surrogate

Mutations in the conserved AAACA sequence of the poliovirus cre element directly correlate with reduced in vitro VPg uridylylation and impaired in vivo viral replication.

Cite This Study

Rieder et al. (2000) studied this question.

synapsesocial.com/papers/6a6f9d9e75498292b708fe14https://doi.org/10.1128/jvi.74.22.10371-10380.2000
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Functional and genetic plasticities of the poliovirus genome: quasi-infectious RNAs modified in the 5'-untranslated region yield a variety of pseudorevertants1993 · 64 citations
  2. 2Interaction between the 5'-terminal cloverleaf and 3AB/3CDpro of poliovirus is essential for RNA replication1995 · 197 citations
  3. 3The Genome-Linked Protein of Picornaviruses1980 · 28 citations
  4. 4Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosine1978 · 244 citations
  5. 5Polioviruses containing picornavirus type 1 and/ortype 2 internal ribosomal entry site elements: genetic hybrids and theexpression of a foreign gene.1994 · 110 citations