November 15, 2000Journal of VirologyOpen Access

Identification of an RNA Hairpin in Poliovirus RNA That Serves as the Primary Template in the In Vitro Uridylylation of VPg

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Population

In vitro models using poliovirus RNA transcripts, synthetic VPg, purified poliovirus RNA polymerase 3Dpol…

Comparison

cre RNA hairpin template and purified viral… vs Other RNA templates including poly, 3'NTR-poly…

Design

Preclinical

Authors

APAniko V. PaulState University of New York ERElizabeth RiederNational Agricultural Statistics Service DKDong Wook KimHanyang University

Discussion

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Implication

May inform picornavirus antiviral design; leaves open in vivo relevance and therapeutic translation.

Structured PICO

Population

In vitro models using poliovirus RNA transcripts, synthetic VPg, purified poliovirus RNA polymerase 3Dpol, and HeLa cell extracts

Intervention

cre(2C) RNA hairpin template and purified viral protein 3CDpro

Comparator

Other RNA templates including poly(A), 3'NTR-poly(A), cloverleaf RNAs, tRNA, and IRES SLII

Outcome

In vitro synthesis of VPgpU(pU) (uridylylation of VPg)surrogate

The study identifies a specific RNA hairpin in the poliovirus genome that acts as the primary template for VPg uridylylation, elucidating a critical step in viral minus-strand RNA synthesis.

Cite This Study

Paul et al. (2000) studied this question.

synapsesocial.com/papers/6a7fa517feefc34aa44222b8 https://doi.org/10.1128/jvi.74.22.10359-10370.2000

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Identification of an RNA Hairpin in Poliovirus RNA That Serves as the Primary Template in the In Vitro Uridylylation of VPg

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Identification of an RNA Hairpin in Poliovirus RNA That Serves as the Primary Template in the In Vitro Uridylylation of VPg

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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