November 1, 2003Journal of Biological ChemistryOpen Access

A “Slide-back” Mechanism for the Initiation of Protein-primed RNA Synthesis by the RNA Polymerase of Poliovirus

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Population

In vitro assay utilizing purified viral proteins, synthetic VPg, UTP, Mg2+, and template RNAs (poliovirus…

Design

Preclinical

Authors

APAniko V. PaulState University of New York JMJoAnn MugaveroStony Brook University ERElizabeth RiederNational Agricultural Statistics Service

Discussion

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Implication

No immediate clinical implications; leaves open whether this mechanism informs antiviral targeting in picornaviruses.

Structured PICO

Population

In vitro assay utilizing purified viral proteins (RNA polymerase 3Dpol, viral protein 3CDpro), synthetic VPg, UTP, Mg2+, and template RNAs (poliovirus RNA or transcripts of a small RNA hairpin cre(2C))

Intervention

Mutant template RNAs and mutations in the 5AAA7 sequence of cre(2C)

Outcome

Initiation site of RNA synthesis, mechanism of VPgpUpU synthesis, viral growth, RNA replication, and 2CATPase protein activity

The study elucidates a 'slide-back' mechanism for the initiation of poliovirus RNA replication, providing insights into viral RNA polymerase function.

Cite This Study

Paul et al. (2003) studied this question.

synapsesocial.com/papers/6a891d18d5f4e697bb13243f https://doi.org/10.1074/jbc.m307441200

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A “Slide-back” Mechanism for the Initiation of Protein-primed RNA Synthesis by the RNA Polymerase of Poliovirus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

A “Slide-back” Mechanism for the Initiation of Protein-primed RNA Synthesis by the RNA Polymerase of Poliovirus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

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Also Consider