December 26, 2002Journal of Virology

Biochemical and Genetic Studies of the VPg Uridylylation Reaction Catalyzed by the RNA Polymerase of Poliovirus

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Population

In vitro assays using synthetic VPg, purified RNA polymerase 3Dpol, UTP, divalent cations, poly template…

Comparison

Mutations in poliovirus VPg and substitution… vs Wild-type poliovirus VPg

Design

Preclinical

Authors

APAniko V. PaulState University of New York JPJ. PetersState University of New York JMJoAnn MugaveroStony Brook University

Discussion

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Implication

Confirms VPg uridylylation essentiality in poliovirus replication; leaves open Mn2+-targeted antivirals as a research direction.

Structured PICO

Population

In vitro assays using synthetic VPg, purified RNA polymerase 3Dpol, UTP, divalent cations, poly(A) template, cre(2C) RNA, HeLa cells, and yeast two-hybrid system

Intervention

Mutations in poliovirus VPg (e.g., Y3T T4Y, G5P, P7A, N8A, K10A) and substitution with human rhinovirus (HRV) VPgs

Comparator

Wild-type poliovirus VPg

Outcome

VPg uridylylation activity, viral growth/viability, and protein-protein interactionsurrogate

Mutations in poliovirus VPg that impair in vitro uridylylation abolish viral growth, and the RNA polymerase strongly prefers Mn2+ as a cofactor.

Cite This Study

Paul et al. (2002) studied this question.

synapsesocial.com/papers/6a7fa517feefc34aa44222b3 https://doi.org/10.1128/jvi.77.2.891-904.2003

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Biochemical and Genetic Studies of the VPg Uridylylation Reaction Catalyzed by the RNA Polymerase of Poliovirus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Biochemical and Genetic Studies of the VPg Uridylylation Reaction Catalyzed by the RNA Polymerase of Poliovirus

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider