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June 13, 2003Journal of VirologyOpen Access

Viability of Poliovirus/Rhinovirus VPg Chimeric Viruses and Identification of an Amino Acid Residue in the VPg Gene Critical for Viral RNA Replication

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Population

H1-HeLa cells, E. coli BL21(DE3) cells, poliovirus (PV), and human rhinovirus 16 (HRV16) models

Comparison

Construction of chimeric viruses and… vs Wild-type poliovirus and human rhinovirus 16

Design

Preclinical

Authors

ICI. Wayne CheneyPublic Health Agency of CanadaSNSuhaila NaimUniversity of California, Los AngelesJSJae Hoon ShimYonsei University

Discussion

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Implication

No immediate clinical implications; leaves open VPg as a target for enterovirus attenuation or antiviral strategies pending human validation.

Structured PICO

P
Population
H1-HeLa cells, E. coli BL21(DE3) cells, poliovirus (PV), and human rhinovirus 16 (HRV16) models
I
Intervention
Construction of chimeric viruses (PV/R16-VPg and R16/PV-VPg) and introduction of E6V mutation
C
Comparator
Wild-type poliovirus (PV) and human rhinovirus 16 (HRV16)
O
Outcome
Viral RNA replication capability and infectious virus particle production

A single amino acid mutation (E6V) in the VPg gene is critical for viral RNA replication in poliovirus/rhinovirus chimeras.

Cite This Study

Cheney et al. (2003) studied this question.

synapsesocial.com/papers/6a7fa517feefc34aa44222b1https://doi.org/10.1128/jvi.77.13.7434-7443.2003
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Also Consider

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  1. 1Biochemical and Genetic Studies of the Initiation of Human Rhinovirus 2 RNA Replication: Purification and Enzymatic Analysis of the RNA-Dependent RNA Polymerase 3D pol2001 · 28 citations
  2. 2Genetic and Biochemical Studies of Poliovirus cis -Acting Replication Element cre in Relation to VPg Uridylylation2000 · 170 citations
  3. 3Characterization of poliovirus clones containing lethal and nonlethal mutations in the genome-linked protein VPg1990 · 60 citations
  4. 4Structure-Function Relationships of the RNA-dependent RNA Polymerase from Poliovirus (3Dpol)2002 · 74 citations
  5. 5Capsid coding sequence is required for efficient replication of human rhinovirus 14 RNA1996 · 108 citations