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April 11, 2003Journal of Virology

Functional Dissection of a Poliovirus cis -Acting Replication Element PV- cre (2C): Analysis of Single- and Dual- cre Viral Genomes and Proteins That Bind Specifically to PV- cre RNA

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Population

HeLa R19 cell monolayers and Escherichia coli expressing poliovirus RNA polymerase and viral proteins, using…

Comparison

Site-directed mutagenesis of the cis replication… vs Wild-type poliovirus genomes and replicons.

Design

Preclinical

Authors

APAniko V. PaulState University of New YorkEWEckard WimmerCalifornia Institute of TechnologyERElizabeth RiederNational Agricultural Statistics Service

Discussion

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Overview

Minimal cre suffices for VPg uridylylation in vitro; leaves open whether cre relocation informs picornavirus antiviral or vaccine strategies.

Structured PICO

P
Population
HeLa R19 cell monolayers and Escherichia coli expressing poliovirus RNA polymerase and viral proteins, using poliovirus (PV1) full-length genomes and replicons (P/L).
I
Intervention
Site-directed mutagenesis of the cis replication element (cre) in the 2C(ATPase) coding region and insertion of cre elements at different genomic locations.
C
Comparator
Wild-type poliovirus genomes and replicons.
O
Outcome
Viral replication (measured by luciferase activity and plaque assay) and VPg uridylylation in vitro.surrogate

This basic science study identifies the minimal RNA sequence required for poliovirus VPg uridylylation and demonstrates that the genomic location of the cre element does not affect RNA infectivity.

Cite This Study

Paul et al. (2003) studied this question.

synapsesocial.com/papers/6a7fa517feefc34aa44222b2https://doi.org/10.1128/jvi.77.9.5152-5166.2003
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Also Consider

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  4. 4Interaction between the 5'-terminal cloverleaf and 3AB/3CDpro of poliovirus is essential for RNA replication1995 · 197 citations
  5. 5Structure-Function Relationships of the RNA-dependent RNA Polymerase from Poliovirus (3Dpol)2002 · 74 citations