What question did this study set out to answer?

To develop and validate an automated and manual spectrophotometric assay using the ABTS.+ radical cation to measure total antioxidant capacity in body fluids and assess antioxidant status in premature infants.

April 1, 1993Clinical Science

A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates

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To develop and validate an automated and manual spectrophotometric assay using the ABTS.+ radical cation to measure total antioxidant capacity in body fluids and assess antioxidant status in premature infants.
Developed automated centrifugal analyser and manual spectrophotometric protocols measuring the absorbance reduction of the ABTS.+ radical cation relative to a 1.0 mmol/l Trolox reference standard.
Calibrated and validated the assay across individual physiological antioxidants, radical-scavenging drugs, and plasma samples from an adult reference population.
Applied the optimized measurement technique to investigate total plasma antioxidant capacity in premature and full-term neonates.
Established an antioxidant reactivity ranking for physiological compounds and therapeutic radical scavengers standardized against Trolox.
Demonstrated that plasma total antioxidant capacity can be reliably quantified and is compromised in premature neonates compared to reference baselines.

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Implication

Assay validation study demonstrates an ABTS-based method to measure compromised total antioxidant capacity in premature neonates, highlighting vulnerability to oxidative stress.

Cite This Study

Miller et al. (1993) studied this question.

synapsesocial.com/papers/69d6c882a0177bf533ed9013 https://doi.org/10.1042/cs0840407

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Implication

Assay validation study demonstrates an ABTS-based method to measure compromised total antioxidant capacity in premature neonates, highlighting vulnerability to oxidative stress.

A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates

Key Points

Discussion

Implication

Cite This Study

A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates

Key Points

Discussion

Implication

Cite This Study

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