April 1, 1993Clinical Science
A Novel Method for Measuring Antioxidant Capacity and its Application to Monitoring the Antioxidant Status in Premature Neonates
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Discussion
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Implication
Assay validation study demonstrates an ABTS-based method to measure compromised total antioxidant capacity in premature neonates, highlighting vulnerability to oxidative stress.
Key Points
- To develop and validate an automated and manual spectrophotometric assay using the ABTS.+ radical cation to measure total antioxidant capacity in body fluids and assess antioxidant status in premature infants.
- Developed automated centrifugal analyser and manual spectrophotometric protocols measuring the absorbance reduction of the ABTS.+ radical cation relative to a 1.0 mmol/l Trolox reference standard.
- Calibrated and validated the assay across individual physiological antioxidants, radical-scavenging drugs, and plasma samples from an adult reference population.
- Applied the optimized measurement technique to investigate total plasma antioxidant capacity in premature and full-term neonates.
- Established an antioxidant reactivity ranking for physiological compounds and therapeutic radical scavengers standardized against Trolox.
- Demonstrated that plasma total antioxidant capacity can be reliably quantified and is compromised in premature neonates compared to reference baselines.
Cite This Study
Miller et al. (1993) studied this question.