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Characterize the photomorphogenic factors UVR1, HY5, and HYH in grapevine (Vitis vinifera) and determine their molecular roles in UV-B signalling and environmental flavonol accumulation.

August 19, 2016Journal of Experimental BotanyOpen Access

The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment

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Characterize the photomorphogenic factors UVR1, HY5, and HYH in grapevine (Vitis vinifera) and determine their molecular roles in UV-B signalling and environmental flavonol accumulation.
Cloned grapevine UVR1, HY5, and HYH, performing functional complementation assays in Arabidopsis uvr8 and hy5 mutants.
Integrated microarray and RNA sequencing data with promoter motif inspection and transient expression assays to identify downstream targets.
Assessed gene expression patterns and flavonol levels in in vitro plantlets and grape berries exposed to high or low UV-B, shading, and temperature changes.
Grapevine UVR1, HY5, and HYH rescued Arabidopsis mutant phenotypes, restoring radiation-dependent morphology and secondary metabolic responses.
HY5 and HYH displayed cooperative and partially redundant roles, directly up-regulating flavonol-related genes through conserved promoter binding sites.
Berry UVR1 expression was altered by shading and temperature rather than UV-B exposure, while HY5 and HYH drove flavonol accumulation across developmental stages under both high and low UV-B.

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Implication

Experimental study demonstrates that UVR1, HY5, and HYH orchestrate UV-B responses and flavonol accumulation in grapevine, indicating distinct environmental regulation across fruit developmental...

Cite This Study

Loyola et al. (2016) studied this question.

synapsesocial.com/papers/69d7246e8a0e2c5879bef8ae https://doi.org/10.1093/jxb/erw307

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Implication

Experimental study demonstrates that UVR1, HY5, and HYH orchestrate UV-B responses and flavonol accumulation in grapevine, indicating distinct environmental regulation across fruit developmental...

The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment

Key Points

Discussion

Implication

Cite This Study

The photomorphogenic factors UV-B RECEPTOR 1, ELONGATED HYPOCOTYL 5, and HY5 HOMOLOGUE are part of the UV-B signalling pathway in grapevine and mediate flavonol accumulation in response to the environment

Key Points

Discussion

Implication

Cite This Study

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