What question did this study set out to answer?

To determine whether CRISPR loci and associated cas genes provide acquired immunity against bacteriophages in prokaryotes.

March 22, 2007Science

CRISPR Provides Acquired Resistance Against Viruses in Prokaryotes

View Full Paper

Ask AI

Bookmark

Authors

Experimental study demonstrates acquired phage resistance in bacteria, revealing sequence-specific CRISPR immunity.

To determine whether CRISPR loci and associated cas genes provide acquired immunity against bacteriophages in prokaryotes.
Challenged bacterial strains with bacteriophages and monitored changes in CRISPR genomic loci.
Experimentally added and removed specific phage-derived spacer sequences within the CRISPR array to evaluate resistance phenotypes.
Bacteria integrated novel spacer sequences directly derived from infecting phage genomes following viral challenge.
Alteration of specific CRISPR spacers directly modified phage-resistance phenotypes based on sequence similarity between spacers and target phage genomes.

Barrangou et al. (2007) studied this question.

View Full Paper

Ask AI

Bookmark