January 8, 2008Journal of Biological ChemistryOpen Access

Palladin Is an Actin Cross-linking Protein That Uses Immunoglobulin-like Domains to Bind Filamentous Actin

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Population

Purified palladin and filamentous actin (F-actin) in vitro

Design

Preclinical

Authors

RDRichard D.S. DixonUniversity of North Carolina at Chapel Hill DADaniel ArnemanUniversity of North Carolina at Chapel Hill ARAndrew RachlinUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Palladin Ig domains mediate F-actin binding and bundling in vitro; leaves open roles in cell morphology and requires in vivo validation.

Structured PICO

Population

Purified palladin and filamentous actin (F-actin) in vitro

Intervention

Biochemical and microscopic assays (co-sedimentation, differential sedimentation, fluorescence and electron microscopy) using purified palladin and its fragments

Outcome

Direct binding and cross-linking of palladin to F-actinsurrogate

Palladin directly binds and cross-links filamentous actin via specific immunoglobulin-like domains, suggesting a role in generating specialized actin-based cell morphologies.

Cite This Study

Dixon et al. (2008) studied this question.

synapsesocial.com/papers/69d72b24b815ed77c2bef1db https://doi.org/10.1074/jbc.m707694200

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