What question did this study set out to answer?

To identify the transcriptional coactivators that partner with mammalian TEAD/TEF transcription factors and characterize how their interaction controls gene expression.

May 15, 2001Genes & DevelopmentOpen Access

TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm

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Authors

AVAlex VassilevNational Institutes of Health KKKotaro KanekoThe University of Tokyo HSHongjun ShuZhejiang Ocean University

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Implication

Molecular study demonstrates that TEAD transcription factors require the coactivator YAP65 for gene activation in mouse cells, suggesting YAP65 mediates mitogenic signaling via TEAD.

Key Points

To identify the transcriptional coactivators that partner with mammalian TEAD/TEF transcription factors and characterize how their interaction controls gene expression.
Purified TEAD-2/TEF-4 protein complexes from mouse cells to isolate and identify associated regulatory proteins.
Assayed protein-protein interactions and transcriptional activation across all four TEAD family members and YAP65 in mouse lymphocytic cells, including MPC11 cells.
Analyzed the subcellular localization of TEAD and YAP65 along with YAP65 binding to the cytoplasmic localization protein 14-3-3.
YAP65 bound directly through its amino terminus to the carboxyl terminus of all four TEAD proteins, providing the essential acidic activation domain required for TEAD-dependent transcription.
Expression of YAP in MPC11 lymphocytic cells yielded up to a 300-fold induction of TEAD transcriptional activity, whereas TEAD overexpression squelched YAP activity.
TEAD proteins localized primarily to the cell nucleus, whereas excess YAP65 was sequestered in the cytoplasm in complexes with 14-3-3.

Cite This Study

Vassilev et al. (2001) studied this question.

synapsesocial.com/papers/69d74c77b1cb92dd1bb8a55f https://doi.org/10.1101/gad.888601

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Implication

Molecular study demonstrates that TEAD transcription factors require the coactivator YAP65 for gene activation in mouse cells, suggesting YAP65 mediates mitogenic signaling via TEAD.

TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm

Discussion

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Implication

Key Points

Cite This Study

TEAD/TEF transcription factors utilize the activation domain of YAP65, a Src/Yes-associated protein localized in the cytoplasm

Discussion

Member takes

Implication

Key Points

Cite This Study

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