Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling

Irene Georgakoudi; Melissa C. Skala; Kyle P. Quinn; Chiara Stringari; Janet E. Sorrells; Ahmed A. Heikal; Lin Z. Li; He N. Xu; Sixian You; Alex J. Walsh; Rupsa Datta; Kayvan Samimi; Amani A. Gillette; Kevin W. Eliceiri; Mihaela Balu; Stephen A. Boppart; Michelle A. Digman; Kylie R. Dunning; Conor L. Evans; Alba Alfonso‐García; Jessica P. Houston; Wonsang Hwang; Matthew Lindley; Xingde Li; Zhiyi Liu; Laura Marcu; Sangeeta Murugkar; Michael G. Nichols; Raluca Niesner; Sapun H. Parekh; Narasimhan Rajaram; Suman Ranjit; K. Robert Shen; Lingyan Shi; Belén Torrado; Alexander Vallmitjana; Michael Evers; Roger J. Zemp

doi:10.1117/1.jbo.30.s2.s23901

Key Points

Establish a consistent and practical framework for label-free optical metabolic imaging to enhance accuracy and reproducibility across biomedical research and clinical translation.
Formulated consensus-based operational and reporting recommendations for cellular label-free optical metabolic imaging.
Defines standard guidelines to enable robust and reliable data comparisons across disparate imaging platforms and research studies.
Provides practical benchmarks to accelerate the clinical adoption and translation of cellular optical metabolic profiling technologies.

Implication

Expert consensus reveals a standardized framework for label-free optical metabolic imaging in cellular systems, supporting clinical translation.

Abstract

We recommend a consistent, practical framework for reproducible, label-free, optical metabolic imaging, facilitating robust comparisons across studies and supporting the broader adoption of optical metabolic imaging technologies for biomedical research and clinical translation.

Discussion

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Cite This Study

Georgakoudi et al. (2025) studied this question.

synapsesocial.com/papers/69d768e1b843b2be9948f9bd https://doi.org/10.1117/1.jbo.30.s2.s23901

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Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling

Key Points

Implication

Abstract

Discussion

Cite This Study

Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling

Key Points

Implication

Abstract

Discussion

Cite This Study

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