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September 10, 2025Biomedical Optics ExpressOpen Access

Light and metabolism: label-free optical imaging of metabolic activities in biological systems Invited

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Authors

LMLuca MenozziZLZhi LiSCSeongwook Choi

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Overview

This review discusses label-free optical imaging's role in metabolic activities and diagnosis, highlighting its importance in various health fields.

Key Points

  • Label-free optical imaging reveals deeper insights into metabolic dynamics, enhancing understanding of cellular functions and disease states.
  • Photoacoustic imaging quantifies hemoglobin oxygenation and glucose levels, while fluorescence techniques assess cellular energy metabolism through NADH and FAD.
  • Interferometric methods like OCT and SHG provide vital information about morphological changes and tissue structures, aiding in clinical diagnostics.
  • Advancements in imaging techniques improve penetration depth and clinical applicability, highlighting their potential in oncology, neurology, and dermatology.

Cite This Study

Menozzi et al. (2025) studied this question.

synapsesocial.com/papers/68c1d5fe54b1d3bfb60f93cfhttps://doi.org/10.1364/boe.572304
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  1. 1Advances in label-free multimodal multiphoton imaging of living tissues2024
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  4. 4Consensus guidelines for cellular label-free optical metabolic imaging: ensuring accuracy and reproducibility in metabolic profiling2025
  5. 5Towards the development of standard nomenclature and calibration standards for optical, label-free, fluorescence based metabolic imaging2024