Label-free, multi-scale imaging of mitochondrial and metabolic function in living tissues

This presentation will highlight the unique opportunities to assess metabolic and mitochondrial function and/or dysfunction afforded by label-free, two photon imaging. The high resolution of two-photon images enables unique assessments of the spatiotemporal heterogeneity of metabolic and mitochondrial dysfunction, which is recognized increasingly as a significant driver of events that determine the development and/or treatment response of numerous diseases. The combined use of readouts from fluorescence intensity and lifetime images yields sensitivity and specificity to metabolic function changes. Examples to highlight such measurements in the context of cancer, vitiligo, and neurodegeneration will be presented.