What question did this study set out to answer?

To evaluate candidate DNA regions across the fungal kingdom and designate an optimal universal DNA barcode marker for fungal species identification.

March 27, 2012Proceedings of the National Academy of SciencesOpen Access

Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

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Authors

CSConrad L. SchochNational Institutes of Health KSKeith A. SeifertUniversity of Northern Iowa SHSabine M. HuhndorfField Museum of Natural History

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Implication

Multilaboratory comparative study demonstrates superior identification capability of the internal transcribed spacer region in fungi, establishing a universal standard for fungal DNA barcoding.

Key Points

To evaluate candidate DNA regions across the fungal kingdom and designate an optimal universal DNA barcode marker for fungal species identification.
A multinational, multilaboratory consortium evaluated six potential DNA barcode regions across diverse fungal lineages.
Markers tested included three nuclear ribosomal RNA regions (small subunit, large subunit, and internal transcribed spacer) and three protein-coding genes (two RNA polymerase II subunits and minichromosome maintenance protein).
The internal transcribed spacer (ITS) region demonstrated the highest probability of successful species identification across the broadest fungal taxa, supported by a distinct barcode gap between inter- and intraspecific variation.
Protein-coding regions displayed higher identification accuracy but were eliminated as universal candidates due to low PCR amplification and sequencing success.
The nuclear ribosomal large subunit provided superior resolution in specific clades such as early diverging lineages and ascomycete yeasts, whereas the small subunit showed poor species-level resolution.

Cite This Study

Schoch et al. (2012) studied this question.

synapsesocial.com/papers/69d807f405ee2ba81dbeec21 https://doi.org/10.1073/pnas.1117018109

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Implication

Multilaboratory comparative study demonstrates superior identification capability of the internal transcribed spacer region in fungi, establishing a universal standard for fungal DNA barcoding.

Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

Discussion

Member takes

Implication

Key Points

Cite This Study

Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi

Discussion

Member takes

Implication

Key Points

Cite This Study

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