Why the study?
Does nested RT-PCR improve the detection limit of group A rotaviruses compared to standard RT-PCR?
Population
Rotavirus strains of human, bovine, porcine, canine, feline, equine, and ovine origin, and faecal specimens
Comparison
Nested reverse transcriptase-polymerase chain… vs Standard RT-PCR and other non-PCR detection…
Design
Preclinical
Authors
Loading...
May enhance rotavirus detection in research labs; leaves open clinical veterinary use pending validation.
Does nested RT-PCR improve the detection limit of group A rotaviruses compared to standard RT-PCR?
A nested RT-PCR assay targeting gene segment 6 provides a highly sensitive diagnostic tool for detecting group A rotaviruses across multiple species.
Elschner et al. (2002) studied this question.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: