What question did this study set out to answer?

Determine bacterial diversity in human subgingival plaque using culture-independent 16S rDNA sequencing across healthy and diseased periodontal states.

June 15, 2001Journal of BacteriologyOpen Access

Bacterial Diversity in Human Subgingival Plaque

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Authors

BPBruce J. PasterStatens Serum Institut SBSusan K. Boches JGJamie L. GalvinFlorida Fish and Wildlife Conservation Commission

Discussion

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Implication

Molecular profiling uncovers extensive uncultivated bacterial diversity across subgingival plaque in healthy and periodontally diseased subjects, highlighting novel phylotypes in oral pathogenesis.

Key Points

Determine bacterial diversity in human subgingival plaque using culture-independent 16S rDNA sequencing across healthy and diseased periodontal states.
Analyzed subgingival plaque from healthy subjects and those with refractory, adult, HIV-associated periodontitis, or acute necrotizing ulcerative gingivitis.
PCR amplified 16S rDNA from isolated DNA with broad and selective primers, cloning inserts into Escherichia coli for sequencing (N=2,522 clones).
Compared nearly complete 1,500-base sequences against reference databases to classify known species and identify novel phylotypes.
Identified 347 species or phylotypes across 9 bacterial phyla among 2,522 clones, with approximately 60% corresponding to 132 known species and 40% representing 215 novel phylotypes.
Calculated an estimated subgingival richness of 415 species including 68 projected unobserved species, supporting a broader oral diversity estimate of approximately 500 species.
Established that conventional periodontal pathogens typically constituted minor plaque components, while specific novel phylotypes and two uncultivable phyla occurred exclusively in diseased sites.

Cite This Study

Paster et al. (2001) studied this question.

synapsesocial.com/papers/69d8179952654bb436d17db7 https://doi.org/10.1128/jb.183.12.3770-3783.2001

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June 15, 2001Journal of BacteriologyOpen Access

Bacterial Diversity in Human Subgingival Plaque

View Full Paper

Ask AI

Bookmark

Authors

BPBruce J. PasterStatens Serum Institut SBSusan K. Boches JGJamie L. GalvinFlorida Fish and Wildlife Conservation Commission

Discussion

Member takes

Implication

Molecular profiling uncovers extensive uncultivated bacterial diversity across subgingival plaque in healthy and periodontally diseased subjects, highlighting novel phylotypes in oral pathogenesis.

Key Points

Determine bacterial diversity in human subgingival plaque using culture-independent 16S rDNA sequencing across healthy and diseased periodontal states.
Analyzed subgingival plaque from healthy subjects and those with refractory, adult, HIV-associated periodontitis, or acute necrotizing ulcerative gingivitis.
PCR amplified 16S rDNA from isolated DNA with broad and selective primers, cloning inserts into Escherichia coli for sequencing (N=2,522 clones).
Compared nearly complete 1,500-base sequences against reference databases to classify known species and identify novel phylotypes.
Identified 347 species or phylotypes across 9 bacterial phyla among 2,522 clones, with approximately 60% corresponding to 132 known species and 40% representing 215 novel phylotypes.
Calculated an estimated subgingival richness of 415 species including 68 projected unobserved species, supporting a broader oral diversity estimate of approximately 500 species.
Established that conventional periodontal pathogens typically constituted minor plaque components, while specific novel phylotypes and two uncultivable phyla occurred exclusively in diseased sites.

Cite This Study

Paster et al. (2001) studied this question.

synapsesocial.com/papers/69d8179952654bb436d17db7 https://doi.org/10.1128/jb.183.12.3770-3783.2001

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