What question did this study set out to answer?

To reconstruct a systematic, comprehensive model of mammalian cellular trajectories spanning gastrulation through organogenesis in vivo.

March 1, 2022Nature GeneticsOpen Access

Systematic reconstruction of cellular trajectories across mouse embryogenesis

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Authors

CQChengxiang QiuDartmouth College JCJunyue CaoRockefeller University BMBeth MartinHoward Hughes Medical Institute

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Implication

Single-cell transcriptomic analysis reveals developmental trajectories across mouse embryogenesis, highlighting key transcription factors governing cell specification.

Key Points

To reconstruct a systematic, comprehensive model of mammalian cellular trajectories spanning gastrulation through organogenesis in vivo.
Integrated existing single-cell RNA-sequencing datasets spanning mouse gastrulation and organogenesis (E3.5 to E13.5).
Generated new single-nucleus RNA-sequencing data from ~150,000 nuclei of E8.5 mouse embryos staged in one-somite increments.
Constructed an automated directed acyclic graph, Trajectories of Mammalian Embryogenesis (TOME), linking cell states to pseudoancestors and pseudodescendants across 19 stages.
Defined cell states across 19 sequential developmental stages, creating a global graph consistent with established mammalian embryology.
Systematically nominated transcription factor candidates acting as key regulators of lineage-specific cell-type specification.
Identified conserved cell-type homologs across vertebrate evolution using the unified trajectory graph.

Cite This Study

Qiu et al. (2022) studied this question.

synapsesocial.com/papers/69d8aa01a5ecc596b5d1822c https://doi.org/10.1038/s41588-022-01018-x

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Implication

Single-cell transcriptomic analysis reveals developmental trajectories across mouse embryogenesis, highlighting key transcription factors governing cell specification.

Systematic reconstruction of cellular trajectories across mouse embryogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

Systematic reconstruction of cellular trajectories across mouse embryogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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