What question did this study set out to answer?

To evaluate the cell-type-specific expression of SARS-CoV-2 entry genes (ACE2, TMPRSS2, and CTSL) across multiple human tissues and assess how demographic factors influence these expression levels.

March 1, 2021Nature MedicineOpen Access

Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics

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Authors

CMChristoph MuusBroad Institute MLMalte D. LueckenHelmholtz Zentrum München GEGökcen EraslanBroad Institute

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Implication

Meta-analysis reveals increased SARS-CoV-2 entry gene expression in older males and specific epithelial cells, highlighting demographic risks.

Key Points

To evaluate the cell-type-specific expression of SARS-CoV-2 entry genes (ACE2, TMPRSS2, and CTSL) across multiple human tissues and assess how demographic factors influence these expression levels.
Surveyed cell-type-specific expression profiles across 107 single-cell RNA-sequencing studies covering various tissues.
Conducted a single-cell meta-analysis of 31 lung datasets comprising 1,320,896 cells from 377 nasal, airway, and lung parenchyma samples across 228 individuals to evaluate associations with age, sex, and smoking status.
ACE2, TMPRSS2, and CTSL were co-expressed primarily in specific respiratory epithelial subsets across nasal passages, airways, and alveoli, as well as in COVID-19-relevant non-respiratory organs.
Expression of SARS-CoV-2 entry factors increased with older age and male sex, notably within airway secretory cells and alveolar type 2 cells.
ACE2+TMPRSS2+ cells shared gene expression programs across nasal, lung, and gut tissues involving epithelial-macrophage cross-talk and inflammatory pathways, including interleukin-6, interleukin-1, tumor necrosis factor, and complement cascades.

Cite This Study

Muus et al. (2021) studied this question.

synapsesocial.com/papers/69d8aa01a5ecc596b5d1822e https://doi.org/10.1038/s41591-020-01227-z

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Implication

Meta-analysis reveals increased SARS-CoV-2 entry gene expression in older males and specific epithelial cells, highlighting demographic risks.

Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics

Discussion

Member takes

Implication

Key Points

Cite This Study

Single-cell meta-analysis of SARS-CoV-2 entry genes across tissues and demographics

Discussion

Member takes

Implication

Key Points

Cite This Study

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