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Experimental imaging demonstrates real-time lipid droplet motility along microtubules using LD540, highlighting its utility for multicolor live cell microscopy.
A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.
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Spandl et al. (2009) studied this question.
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