What question did this study set out to answer?

To characterize the nucleotide sequence and chromosomal integration locus of insertion element IS987 in Mycobacterium bovis BCG and evaluate its distribution across related mycobacterial species.

August 1, 1991Infection and ImmunityOpen Access

Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains

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Authors

PHPeter W. M. HermansUniversity Medical Center DSDick van SoolingenStatens Serum Institut EBElisabeth M. BikProtein Metrics (United States)

Discussion

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Implication

Molecular analysis reveals direct repeat loci hosting insertion sequences in Mycobacterium tuberculosis complex strains, suggesting a preferential chromosomal integration hot spot.

Key Points

To characterize the nucleotide sequence and chromosomal integration locus of insertion element IS987 in Mycobacterium bovis BCG and evaluate its distribution across related mycobacterial species.
Cloned and sequenced the single-copy IS987 insertion element along with its flanking regions from Mycobacterium bovis BCG.
Used polymerase chain reaction to analyze chromosomal direct repeat clusters in M. bovis BCG, nine non-tuberculosis mycobacterial species, and 14 clinical Mycobacterium tuberculosis strains.
IS987 was virtually identical to IS986 from M. tuberculosis and was integrated into the 30th repeat of a cluster containing 49 direct repeats of 36 base pairs separated by 35 to 41 base-pair spacers.
Direct repeat sequences were uniquely present in Mycobacterium tuberculosis complex members and absent from all nine other tested mycobacterial species.
Among 14 M. tuberculosis strains, eight contained one insertion element in the direct repeat region, five contained two, and one contained none, demonstrating length and structural polymorphism.

Cite This Study

Hermans et al. (1991) studied this question.

synapsesocial.com/papers/69d8ef6c8c03fbaff8bf01dd https://doi.org/10.1128/iai.59.8.2695-2705.1991

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August 1, 1991Infection and ImmunityOpen Access

Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains

View Full Paper

Ask AI

Bookmark

Authors

PHPeter W. M. HermansUniversity Medical Center DSDick van SoolingenStatens Serum Institut EBElisabeth M. BikProtein Metrics (United States)

Discussion

Member takes

Implication

Molecular analysis reveals direct repeat loci hosting insertion sequences in Mycobacterium tuberculosis complex strains, suggesting a preferential chromosomal integration hot spot.

Key Points

To characterize the nucleotide sequence and chromosomal integration locus of insertion element IS987 in Mycobacterium bovis BCG and evaluate its distribution across related mycobacterial species.
Cloned and sequenced the single-copy IS987 insertion element along with its flanking regions from Mycobacterium bovis BCG.
Used polymerase chain reaction to analyze chromosomal direct repeat clusters in M. bovis BCG, nine non-tuberculosis mycobacterial species, and 14 clinical Mycobacterium tuberculosis strains.
IS987 was virtually identical to IS986 from M. tuberculosis and was integrated into the 30th repeat of a cluster containing 49 direct repeats of 36 base pairs separated by 35 to 41 base-pair spacers.
Direct repeat sequences were uniquely present in Mycobacterium tuberculosis complex members and absent from all nine other tested mycobacterial species.
Among 14 M. tuberculosis strains, eight contained one insertion element in the direct repeat region, five contained two, and one contained none, demonstrating length and structural polymorphism.

Cite This Study

Hermans et al. (1991) studied this question.

synapsesocial.com/papers/69d8ef6c8c03fbaff8bf01dd https://doi.org/10.1128/iai.59.8.2695-2705.1991

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