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April 1, 2002Journal of Clinical MicrobiologyOpen Access

Novel IS 6110 Insertion Sites in the Direct Repeat Locus of Mycobacterium tuberculosis Clinical Strains from the St. Petersburg Area of Russia and Evolutionary and Epidemiological Considerations

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Authors

IMIgor MokrousovSaint Petersburg Pasteur InstituteONOlga NarvskayaSaint Petersburg Pasteur InstituteELElena LimeschenkoSaint Petersburg Pasteur Institute

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Implication

Genotyping study reveals novel IS6110 insertion sites in Mycobacterium tuberculosis clinical isolates, highlighting critical diagnostic pitfalls in standard spoligotyping interpretation.

Key Points

  • Identify and characterize novel IS6110 insertion sites within the direct repeat (DR) locus of Mycobacterium tuberculosis clinical isolates.
  • Applied a modified spoligotyping assay incorporating primers specific to both direct repeat (DR) and IS6110 sequences.
  • Analyzed clinical isolates from the St. Petersburg region of Russia representing both Beijing and non-Beijing genotypes.
  • Identified an asymmetrical direct IS6110 insertion in DR37 in two epidemiologically unlinked Beijing family strains, confirming high conservation of the Beijing DR locus.
  • Detected novel asymmetrical insertions in DR38 and DR14 across two distinct non-Beijing strains.
  • Demonstrated that asymmetrical insertions prevent standard spoligotyping hybridization at adjacent spacers, notably affecting the interpretation of signals 37 and 38.

Cite This Study

Mokrousov et al. (2002) studied this question.

synapsesocial.com/papers/6a9464d64efb9d310ac7d0afhttps://doi.org/10.1128/jcm.40.4.1504-1507.2002
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

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  4. 4Spoligotype Database ofMycobacterium tuberculosis: Biogeographic Distribution of Shared Types and Epidemiologic and Phylogenetic Perspectives2001 · 110 citations
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