What question did this study set out to answer?

The aim is to assess the genotoxic and oxidative effects of 2,4-D on Rhinella arenarum tadpoles.

Genotoxic and Biochemical Responses of the Amphibian Rhinella arenarum Under Exposure to the Herbicide 2,4‐Dichlorophenoxyacetic Acid (2,4‐D)

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Evaluates genotoxic and oxidative effects of herbicide exposure in amphibians, suggesting significant environmental implications.

The aim is to assess the genotoxic and oxidative effects of 2,4-D on Rhinella arenarum tadpoles.
Acute exposure of tadpoles to 5 μg/L of 2,4-D under laboratory conditions.
Genotoxicity assessed using the alkaline comet assay for DNA breaks.
Oxidative stress evaluated by measuring catalase and GST activities and reduced glutathione levels.
Significant increases in DNA damage were observed, with 3.32- and 2.92-fold increases at 48 and 96 hours, respectively.
Catalase activity decreased by 43.8% after 96 hours of exposure.
Reduced glutathione levels decreased by 29.8%, indicating oxidative stress induction.

Laborde et al. (2026) studied this question.

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