What question did this study set out to answer?

To identify the primary cell surface receptor responsible for cellular binding and infection by adeno-associated virus type 2 (AAV-2).

February 1, 1998Journal of VirologyOpen Access

Membrane-Associated Heparan Sulfate Proteoglycan Is a Receptor for Adeno-Associated Virus Type 2 Virions

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Authors

CSCandace SummerfordUniversity of North Carolina at Chapel Hill RSR. Jude SamulskiHeart Failure & Transplant

Discussion

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Overview

In vitro biochemical study demonstrates that membrane-associated heparan sulfate proteoglycan mediates adeno-associated virus type 2 infection, highlighting mechanisms for gene therapy vector design.

Key Points

To identify the primary cell surface receptor responsible for cellular binding and infection by adeno-associated virus type 2 (AAV-2).
Assayed AAV-2 attachment and infection in wild-type and glycosaminoglycan-deficient mutant Chinese hamster ovary (CHO) cell lines.
Performed competition experiments using soluble heparin and enzymatic digestion of cell surface heparan sulfate versus chondroitin sulfate.
Soluble heparin competed with cell surface receptors, producing a dose-dependent inhibition of AAV-2 binding and infection.
Enzymatic removal of cell-surface heparan sulfate—but not chondroitin sulfate—markedly diminished viral attachment and infectivity.
Mutant cell lines lacking heparan sulfate proteoglycans exhibited significant impairments in both AAV-2 binding and successful transduction.

Cite This Study

Summerford et al. (1998) studied this question.

synapsesocial.com/papers/69da00e68fbc15f99e6842dc https://doi.org/10.1128/jvi.72.2.1438-1445.1998

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February 1, 1998Journal of VirologyOpen Access

Membrane-Associated Heparan Sulfate Proteoglycan Is a Receptor for Adeno-Associated Virus Type 2 Virions

View Full Paper

Ask AI

Bookmark

Authors

CSCandace SummerfordUniversity of North Carolina at Chapel Hill RSR. Jude SamulskiHeart Failure & Transplant

Discussion

Member takes

Overview

In vitro biochemical study demonstrates that membrane-associated heparan sulfate proteoglycan mediates adeno-associated virus type 2 infection, highlighting mechanisms for gene therapy vector design.

Key Points

To identify the primary cell surface receptor responsible for cellular binding and infection by adeno-associated virus type 2 (AAV-2).
Assayed AAV-2 attachment and infection in wild-type and glycosaminoglycan-deficient mutant Chinese hamster ovary (CHO) cell lines.
Performed competition experiments using soluble heparin and enzymatic digestion of cell surface heparan sulfate versus chondroitin sulfate.
Soluble heparin competed with cell surface receptors, producing a dose-dependent inhibition of AAV-2 binding and infection.
Enzymatic removal of cell-surface heparan sulfate—but not chondroitin sulfate—markedly diminished viral attachment and infectivity.
Mutant cell lines lacking heparan sulfate proteoglycans exhibited significant impairments in both AAV-2 binding and successful transduction.

Cite This Study

Summerford et al. (1998) studied this question.

synapsesocial.com/papers/69da00e68fbc15f99e6842dc https://doi.org/10.1128/jvi.72.2.1438-1445.1998

Also Consider

Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context:

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