What question did this study set out to answer?

To evaluate the diffusion coefficients and mass transfer behavior of various molecular weight substrates moving into and out of Ca-alginate gel beads.

January 1, 1984Biotechnology and Bioengineering

Diffusion characteristics of substrates in Ca‐alginate gel beads

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Authors

HTHideo TanakaKyoto Prefectural University MMMasatoshi MatsumuraIndustrial Research Institute IVI. A. VelikyNational Research Council Canada

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Overview

In vitro experiment reveals size-dependent diffusion limits in Ca-alginate beads, indicating selective matrix permeability for immobilization.

Key Points

To evaluate the diffusion coefficients and mass transfer behavior of various molecular weight substrates moving into and out of Ca-alginate gel beads.
Measured diffusion coefficients (D) of substrates into and out of Ca-alginate beads in well-stirred aqueous solutions.
Tested low molecular weight molecules (glucose, L-tryptophan, alpha-lactoalbumin; MW < 2 x 10^4) and high molecular weight proteins (albumin, gamma-globulin, fibrinogen; MW up to 3.41 x 10^5).
Assessed diffusion variations across different Ca-alginate concentrations in the beads and CaCl2 concentrations used during preparation.
Substrates with MW below 2 x 10^4 diffuse freely into and out of beads with diffusion coefficients matching those in pure water, unaffected by alginate or CaCl2 concentrations.
High molecular weight proteins (MW 6.9 x 10^4 to 3.41 x 10^5) exhibit no inward diffusion from solution, with only albumin partially adsorbing to the bead surface.
Outward diffusion of large proteins occurs at lower diffusion coefficients than in water, and outward transport is restricted more strongly by higher Ca-alginate concentrations than by CaCl2 concentrations.

Cite This Study

Tanaka et al. (1984) studied this question.

synapsesocial.com/papers/69da58730f32475823a3d418 https://doi.org/10.1002/bit.260260111

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January 1, 1984Biotechnology and Bioengineering

Diffusion characteristics of substrates in Ca‐alginate gel beads

View Full Paper

Ask AI

Bookmark

Authors

HTHideo TanakaKyoto Prefectural University MMMasatoshi MatsumuraIndustrial Research Institute IVI. A. VelikyNational Research Council Canada

Discussion

Member takes

Overview

In vitro experiment reveals size-dependent diffusion limits in Ca-alginate beads, indicating selective matrix permeability for immobilization.

Key Points

To evaluate the diffusion coefficients and mass transfer behavior of various molecular weight substrates moving into and out of Ca-alginate gel beads.
Measured diffusion coefficients (D) of substrates into and out of Ca-alginate beads in well-stirred aqueous solutions.
Tested low molecular weight molecules (glucose, L-tryptophan, alpha-lactoalbumin; MW < 2 x 10^4) and high molecular weight proteins (albumin, gamma-globulin, fibrinogen; MW up to 3.41 x 10^5).
Assessed diffusion variations across different Ca-alginate concentrations in the beads and CaCl2 concentrations used during preparation.
Substrates with MW below 2 x 10^4 diffuse freely into and out of beads with diffusion coefficients matching those in pure water, unaffected by alginate or CaCl2 concentrations.
High molecular weight proteins (MW 6.9 x 10^4 to 3.41 x 10^5) exhibit no inward diffusion from solution, with only albumin partially adsorbing to the bead surface.
Outward diffusion of large proteins occurs at lower diffusion coefficients than in water, and outward transport is restricted more strongly by higher Ca-alginate concentrations than by CaCl2 concentrations.

Cite This Study

Tanaka et al. (1984) studied this question.

synapsesocial.com/papers/69da58730f32475823a3d418 https://doi.org/10.1002/bit.260260111

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