October 6, 2003Nucleic Acids ResearchOpen Access

RNA cleaving '10-23' DNAzymes with enhanced stability and activity

View Full Paper

Ask AI

Bookmark

Structured PICO

Population

In vitro model targeting the 5'-non-translated region of human rhinovirus 14 RNA

Intervention

Modified '10-23' DNAzymes (incorporating 3'-3'-inverted thymidine, phosphorothioate linkages, 2'-O-methyl RNA, locked nucleic acids, and modified catalytic core)

Comparator

Unmodified or differently modified DNAzymes

Outcome

Cleavage activity and nuclease stabilitysurrogate

Optimizing nucleotide modifications in the binding arms and catalytic core of '10-23' DNAzymes significantly enhances their RNA cleavage activity and nuclease stability.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Does not support clinical use; leaves open therapeutic testing of optimized DNAzymes in cardiovascular models.

Cite This Study

Steffen Schubert (2003) studied this question.

synapsesocial.com/papers/69da882c0f0ab7a47c835966 https://doi.org/10.1093/nar/gkg791

View Full Paper

Ask AI

Bookmark

RNA cleaving '10-23' DNAzymes with enhanced stability and activity

Structured PICO

Discussion

Implication

Cite This Study

RNA cleaving '10-23' DNAzymes with enhanced stability and activity

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider